Technically, your wallet file is one factor, and the password for opening the wallet is the second factor, since the wallets are non-deterministically generated.
If you're looking for one time passwords, they're based on a "shared secret" and thus only make sense when you're authenticating to a second party with whom you can share a secret, as with a web based wallet.
Multisig transactions will eventually support an additional form of multi factor security, but this is not available yet.